Novel tools for cell line engineering with CRISPR-Cas9

The CRISPR-Cas9 system provides researchers a powerful tool for manipulation of cells to create disease models, reporter cell lines, cell therapy tools, and approaches for novel pathway discoveries. Yet, the steps involved in designing reagents, assessing efficiency of edits, and characterizing cell populations can be time-consuming and laborious. The Invitrogen™ TrueDesign™ Genome Editor software is a one-stop design tool for optimizing CRISPR guide RNAs and donor designs for precise genome editing. The growing lineup of Invitrogen™ TrueTag™ DNA Donor kits offer a full suite of options for successful gene tagging or knockout cell line creation in significantly less time than traditional workflows. The one-step PCR strategy for DNA Donor creation eliminates the need for cloning, and antibiotic resistance markers enable enrichment of up to 100% edited cells.

Learning Objectives

• Utilize free online software to design CRISPR guide RNAs and DNA donors to accomplish genomic insertions, deletions, replacements, and gene tagging
• Discuss how to efficiently tag a protien of interest with a flourescent or epitope tag with no cloning required.
• Create knockout cell lines with less time and effort