4707 Highway 61 N, Suite 250, White Bear Lake, MN 55110
Thu, May 12|
Implementing shallow screening strategies to accelerate microbial strain improvement
We discuss the benefits of creating a higher edit diversity in cell populations while using a shallow screening approach with high-throughput and automated phenotyping systems such as Rapid Fire Mass Spec and plate-based fluorometric assays.
Time & Location
May 12, 2022, 9:00 AM PDT
About the event
The ability to move rapidly through the Design-Generate-Test-Learn (DGTL) cycle is essential for effective forward engineering of microbial phenotypes. The Onyx® platform is an automated CRISPR-based genome editing technology that enables rapid diversity generation. To optimize the DGTL cycle, effective diversity generation must be paired with an appropriate screening strategy for isolated genetic variants. Here we discuss the benefits of creating a higher edit diversity in cell populations while using a shallow screening approach with high-throughput and automated phenotyping systems such as Rapid Fire Mass Spec and plate-based fluorometric assays. We demonstrate the effectiveness of this approach using both metabolic and strain engineering examples, including a 14,000-fold improvement in lysine biosynthesis in E. coli and 4-fold increase in heterologous protein production in S. cerevisiae.
- Review the principles of forward engineering for microbial strain improvement
- Identify goals and develop appropriate strategies for genetic diversity generation
- Demonstrate how to balance diversity generation and screening capacities
- Explain why shallow screening is a beneficial strategy for rapid strain improvement
- Illustrate the effectiveness this approach using metabolic and strain engineering examples